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Coding

Part:BBa_K855003:Design

Designed by: CHU TSZ LONG   Group: iGEM12_HKU_HongKong   (2012-09-24)


coding sequence for pvdQ with histag added to the C-terminal followed by stop codon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1489
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1196
    Illegal XhoI site found at 1594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1489
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1489
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 1329
    Illegal NgoMIV site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1926


Design Notes

It has a PstI site inside the coding sequence of the pvdQ gene


Source

It comes from the genomic sequence of Pseudomonas aeruginosa strain PAO1.

References